Part:BBa_K4201002:Design

gm.cytoGGPPS

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 784
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 784
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 794
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 784
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 784
  Illegal NgoMIV site found at 198
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 1
  Illegal BsaI.rc site found at 971
  Illegal SapI.rc site found at 768
  

Design Notes

Codon optimization for Glycine max (soybean) is a unique design consideration for all of CU Boulder’s coding sequences including our Gm.cytoGGPPS. Codon optimization is the intentional use of specific codons for specific amino acids, dependent on what tRNAs are most abundant in the organism. While codon optimization is a common consideration for synthetic biologists, our sequences are unique for iGEM because they are intended for expression in soybeans. Another design consideration in the removal of chloroplast location tag on the 5’ end of the coding region. This modification forces the Geranylgeranyl-Pyrophosphate Synthase to be expressed in the cytosol and is cited in previous literature².

Source

This Geranylgeranyl-Pyrophosphate Synthase originates from Glycine max. All of our DNA fragments are obtained via de novo synthesis by iGEM sponsors Twist Bioscience and Integrated DNA technologies.

References

1. Zou, D. et al. Production of a novel lycopene-rich soybean food by fermentation with Bacillus amyloliquefaciens. LWT 153, 112551 (2022).
2. De La Peña, R. & Sattely, E. S. Re-routing plant terpene biosynthesis enables momilactone pathway elucidation. Nat. Chem. Biol. 17, 205–212 (2021).